HTRF 96-well low volume plate *To prepare working standard solutions, culture medium can be an alternative the diluent. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. Assay detection requires a microplate reader with. As shown in Figure 1, the interaction between Tag1-SOS1 and Tag2-KRAS-WT is detected by using anti-Tag1-Terbium (HTRF donor) and anti-Tag2-XL665 (HTRF acceptor). As shown in Figure 1, the interaction between Tag1-SOS1 and Tag2-KRAS-WT is detected by using anti-Tag1-Terbium (HTRF donor) and anti-Tag2-XL665 (HTRF acceptor). White 384-well low volume plate Make sure to use the set-up for Eu 3+ Cryptate. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection microplates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. Read & analyse. e. 5 µL. Results for HTRF Reader Control Kit with black and white microplates. Combined with our Kaleido™ workflow software, it. For more information about HTRF. The SpectraMax i3x and SpectraMax iD5 readers can be equipped with an HTRF-certified detection cartridge (i3x) or an HTRF-certified module and filters (iD5) to meet the demanding specifications required for HTRF assays. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. PURCHASE SEPARATELY . Make sure to use the set-up for Eu Cryptate. • User-upgradable microplate reader in under 2 minutes • Array of detection cartridge applications at your fingertips. The two-plate protocol involves culturing cells in a 96-well plate before lysis, then transferring lysates to a low volume detection plate (either HTRF 384-lv or 96-lv plate) before the addition of HTRF Phospho-Rb (Ser780) detection reagents. NC1656382. PURCHASE SEPARATELY . For more information about set-up and compatible HTRF® readers,The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. 47 seconds for a 1536-well plate. Make sure the setup for Eu 3+ Cryptate is used. CLARIOstar is equipped with a specific optical device which enables the measurement of both 620 nm cryptate and 665 nm acceptor emissions. This protocol gives the cells viability and confluence to be monitored. " The PHERAstar FSX instrument is a high quality, reliable, plate reader. 3 and higher include optimized reader settings, as well as data analysis, for faster results. CLARIOstar is equipped with a specific optical device which enables the measurement of. For a list of HTRF-compatible readers and set-up recommendations, please visit our website • Small volume (SV) detection microplates - Use white plate only. When MikroWin opens, if you already have a pre-existing template for. VICTOR® Nivo™ multimode plate reader is the smallest plate reader on the. HTRF Setup. Incubate overnight at RT. The VICTOR Nivo is a HTRF certified microplate reader. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. FOR HTRF CERTIFIED READER. FOR HTRF CERTIFIED READER. HTRF® CertifiedCertified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Concentrate on running your assays instead of running your instrument: the optic modules contain all the filters, dichroics, and more needed to run your assay. Incubate overnight at RT. Synergy H1 offers researchers a choice of monochromators or filters or both detection technologies in one compact unit. Here’s how it works: we come to you with over 30 years of plate reader instrument development experience. FOR HTRF CERTIFIED READER. PHERAstar FS Operating Manual BMG LABTECH Figure 6: Unlocking the plate carrier 3. The FLUOstar Omega is the ideal plate reader for life science applications. You can verify your reader’s ability to detect and use HTRF technology, then easily configure your instrument with its own HTRF setup. Read other about this measurement approach here. Plate 1 plate HTRF 96-well low volume plate *To prepare working standard solutions, culture medium can be an alternative the diluent. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. culture plate. For HTRF certified reader For more information about HTRF® compatible readers and for set- up recommendations, please visit our website. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. 2400 for 665 and 620 with white plate HTRF® Europium cryptate donor / Red acceptor readout. The plate is set up for reading and images are acquired according to specified parameters. culture plate. The EnVision XCite multimode plate reader provides exceptional sensitivity across all detection technologies, while keeping you flexible with monochromator option and full upgradeability. The optical systems use two scanning All the benefits of HTRF with ELISA plate ease of use. The mean and standard deviation. Step 1 Step 2 Step 3 Step 4 Step 5 Step 6. 1. HTRF combines time-resolved fresh detection with FRET and is mainly used for drug screening in high-throughput. Plate 25 μL of cells in. Lysates are then transferred to the assay plate for the detection of total-EGFR by HTRF. FOR HTRF CERTIFIED READER For more. FOR HTRF CERTIFIED READER. The new ligands are divided into four groups according to their chemical structures and/or interactions with YTHDC1 (Table 1 and Fig. FOR HTRF CERTIFIED READER. Data Analysis HTRF®-certified, DLReady, and SpectraMax® DuoLuc™-certified. SpectraMax i3/i3x and Paradigm readers can be equipped with. 41 mg/ml) was obtained from Thermo Fisher Scientific. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. Microplate readerFluorescence microplate readers usually contain a light source, an optical system for the selection of excitation light (i. FOR HTRF CERTIFIED. Lysis buffer 1X for assay protocol on tissues, two-plate assay protocol on adherent cells, and standard dilution. Transcreener® ADP2 TR-FRET assay. PerkinElmer EnVision Multimode Plate Reader. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. + 4 µL HTRF ® pre-mixed antibo dies 2. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detectionThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. 5 μL of Binding buffer 2X. It can also be further streamlined to a one-. 25 µL cells + 5 µL compounds + 10 µL supplemented lysis buffer (4X)* + 4 µL HTRF ® pre-mixed antibodies. Both readers demonstrated their ability to detect IP1 concentrations according to Cisbio’s acceptance criteria. Plate 25 μL of cells in. 5 hours before a further 3 washing steps. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. 45mm 5000µs Delay time Reading time Operation mode 100 µs 400 µs by plate 100 µs 400 µs by plate • This reader only allows high performance HTRF measurement when assays are run in WHITE plates. 96-well culture plateFor each fluorescent ligand concentration, the nonspecific binding was determined by adding a large excess of unlabeled spiperone. For more information about HTRF ®The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Figure 1. Evaluation of the performance of the Spark™ multimode reader for far red fluorescence emission. The plate reader. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. The detection antibody is incubated for 1. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. • Clone your plate data for multiple data reduction optionsExpanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™- certified. Lysis buffer 1X for two-plate assay protocol on adherent cells Determine the amount of lysis buffer needed for the experiment. Seal the plate and incubate 3 hours at RT . For more information about HTRF. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. The SpectraMax M5e Reader offers the additional benefit of being certified for Cisbio Bioassays’ HTRF® technology. HTRF (Homogeneous Time Resolved Fluorescence) is a no-wash technology. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. For more information about HTRF ®The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. The SpectraMax ® iD5 Multi-Mode Microplate Reader is HTRF-certified, with an enhanced TRF detection module and filters optimized for HTRF detection. In particular, these parameters should be entered as below. The laser beam passes through the sample well. CLARIOstar is equipped with a specific optical device which enables the measurement of. Plate 25 μL of cells in. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Lysates are then transferred to the assay plate for the detection of Total H2AX by HTRF ® reagents. 5 µL Prostaglandin E2 . Make sure the setup for Tb Cryptate is used. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. Highly compatible plates for HTRF. Dual monochromators for assay flexibility With SpectraMax Multi-Mode Readers, there is no need to utilize expensive filters to optimize detection levels and background. You can verify your reader’s ability to detect and use HTRF technology, then easily configure your instrument with its own HTRF setup. The assay is then ready to be read on an HTRF compatible or certified microplate reader. Time-resolved fluorescence was measured switch the SpectraMax i3x and SpectraMax iD5 readers using preconfigured protocols in SoftMax® Pro Software (see Tables 2 and 3 for instrument-specific settings). acceptor reagent. STANDARD . Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. Its unique optical design and instant polarizer switching provide the smallest mP standard deviation in any assay. Step 1 Step 2 Step 3 Step 4 Step 5. Tecan Spark multimode microplate reader, with fluorescence Fusion Optics™ meets these challenges and offers everything you need to develop and run advanced biochemical and cell-based fluorescence assays. Lysates are then transferred to the assay plate for the detection of phospho-c-Src (Tyr419) by HTRF. protocol with suspension cells or with a. Incubate overnight at RT. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. PROTOCOL AT A GLANCE. HTRF 96 TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: Add ONE-PLATE ASSAY PROTOCOL: 3h * * Depending on cell lines used, volume of lysis should be optimized. HTRF Microplate Readers. HTRF TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: ONE-PLATE ASSAY PROTOCOL: overnight at RT * * Depending on cell lines used, volume of lysis should be optimized. 1. For more information about HTRF. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. 5 hours before 3 more washing steps. 96-well culture plate• HTRF®-Certified Reader. The HTRF Reader Control Kit provides an easy and straightforward way to assess your reader’s overall performance and can facilitate the identification of potential limitations. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Place the imaging filter cube on top of the LED cube you installed, and use the hex wrench to screw it to the LED cube. FOR HTRF CERTIFIED READER. STANDARD PROTOCOL FOR INHIBITORY ASSAY IN 20 μL FINAL VOLUME Standard Inhibitor Tag1-TNFα Tag2-TNFR1 Anti-Tag1 Eu Cryptate antibody Anti-Tag2 XL665 reagent PPI Europium detection bufferThe perfect microplate reader for everyday biochemical and cell-based assays. This is thanks to a dedicated Alpha Technology laser which outperforms any xenon-based light source by exciting donors at 680 nm. Revvity. NC1286225. (665 nm and 620 nm) on a compatible HTRF ® reader. Make sure the setup for Tb Cryptate is used. For more information about HTRF. Thanks to the unique Optic Module system, Simultaneous Dual Emission, UV/vis spectrometer, AAS System, and dedicated AlphaScreen ® and. The HTRF ratio obtained represents the total binding. Endpoint mode experiments measure each well of the microplate only once. Provide more sensitive and precise results. FOR HTRF CERTIFIED READER For more information about HTRF. FOR HTRF CERTIFIED READER For more information about HTRF. Lysates are then transferred to the assay plate for the detection of total cRaf by HTRF. Read & analyse. The PHERAstar FS is an advanced HTS plate reader designed for all HTRF assays and features the following: All microplate formats up to 3456-well. For multiplexed luminescent and fluorescent assays, using a white plate will support maximum light output signal for the luminescent portion of the assay, but result in higher crosstalk and background for the fluorescent portion of the assay. Spark - Technical Notes. Catalog No. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™- certified. HTRF-compatible SpectraMax Multi-Mode Readers. FOR HTRF CERTIFIED READER. Download Setup. Read & analyse. </p> <p>And, the Enhanced Security software option includes tools to facilitate 21 CFR Part 11 compliance for integration into regulated environments (GxP). 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. Utilizing HTRF (Homogeneous Time-resolved Fluorescence) technology, the assay enables simple and rapid characterization of compound and antibody blockers in a high throughput format. PLATE READER INTRODUCTION Selecting the right plate reader for your lab can be challenging. For more. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. For more. Neocarzinostatin dose-response on Jurkat cells. The Varioskan LUX multimode reader has received HTRF™ certification from Cisbio Bioassays and DLReady™ validation from Promega Corporation. 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies. 55 µL anti-cAMP-Cryptate. Certified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. Make sure the plate reader is turnedon and then open the MikroWin software on the computer. . You can verify your reader’s capacity to detect and use HTRF technology, then easily configure your instrument with its own HTRF setup. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular biology and immunoassays. For more information about HTRF. + 4 µL HTRF pre-mixed antibodies. In this case the Z´(pronounced Z prime) value comes to mind. Incubate 2h at RT Transfer 16 µL supernatant. For more information about microplate recommendations, please visit our website STORAGE AND STABILITY• HTRF®-Certified Reader. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. It can also be further streamlined to a one-. PerkinElmer offers two microplate readers certified for use with HTRF: Envision. The SpectraMax M5e reader offers the additional benefit of being certified for Cisbio Bioassays HTRF® technology. HTRF plate reader. HTRF® Europium cryptate donor / Red acceptor readout Setup recommendations for Tristar 5FOR HTRF CERTIFIED READER. This protocol, HTS designed, allows miniaturization while. 15 / Each of 1; Qty. The reader only allows high performance for the used HTRF measurement when the HTRF is run with white plates. Fluorescence intensity – Fluorescence intensity is one of the most popular detection modes, and there are innumerable fluorophores available for applications such as DNA or protein quantitation, reporter-geneCertified SpectraMax® M5e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. Incubate 4h at RT. Certified for use with HTRF ®, Alpha, LANCE ® or DELFIA ®. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. 10 μL of GLP1 receptor cells were dispensed into wells of. For a list of HTRF-compatible readers and set-up recommendations, please visit . Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. 3. **For more information about HTRF® compatible readers and for set-up recommendations, please visit our website at:ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance,. Customer Transformation Hub. HTRF TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: ONE-PLATE ASSAY PROTOCOL: overnight * * Depending on cell lines used, volume of lysis should be optimized. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. In addition, stacker. You can verify your reader’s ability to detect and use HTRF technology, then easily configure your. Rating: 5. HTRF Signal + 16 µL Standard or Sample 4 µL of pre-mixed IL1β Antibodies ADD READ ANALYSE Incubate overnight* at room temperature *Reading possibe after 2h White 384-well low volume plate Make sure to use the set-up for Eu 3+ Cryptate. Plus . Thanks to HTRF signal stability, the assay plate may be used to check several instruments, or. Visualize cells with your microplate reader Imaging with the SpectraMax MiniMax 300 Imaging Cytometer mirrors the plate reading workflow on the SpectraMax i3x System. ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. Incubate 4h at RT. best fluorescence polarization plate reader on the market. PerkinElmer. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. S. HTRF is a versatile TR-FRET technology developed by Cisbio for detecting biomolecular interactions. • HTRF®-Certified Reader **. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. 7ONE-PLATE ASSAY PROTOCOL: Small volume detection plate. For more information about HTRF. The EnSight<sup>®</sup> multimode plate reader offers HTS Alpha and Ultra-sensitive Luminescence technologies alongside conventional multimode detection technologies. The Firefly reaction is initiated, followed by its quenching and the subsequent initiation of the Renilla reaction. The plate was covered both incubated for one hour at room temperature. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. Plates can be loaded manually or plates can be loaded using 20 or 50-plate free access magazines which fit in a stacker. FOR HTRF CERTIFIED READER For more information about HTRF. at room temperature STEP 4 Remove the plate sealer and read on an HTRF ® compatible reader. This protocol enables the cells' viability and confluence. For more information about microplate recommendations, please visit our website STORAGE AND STABILITYFOR ®READING, AN HTRF -CERTIFIED READER IS NEEDED. TR-FRET was measured on the SpectraMax i3x and iD5 readers (see Table 1 for instrument settings). They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. Plate 25 μL of cells in. microplates can be read on all HTRF certified readers, thus making their implementation in your experiments really easy. The perfect microplate reader for everyday biochemical and cell-based assays, it measures at any wavelength, with top and bottom reading of all technologies. It can also be further streamlined to a one-. For more information about microplate recommendations, please visit our website • Store the kit at -16°C. Reader. This protocol gives the cells viability and confluence to be monitored. The PHERAstar ® FSX and CLARIOstar ® microplate readers offer the best possible performance for AlphaScreen ®, AlphaLISA ®, and AlphaPlex™ assays. 2400 for 665 and 620 with white plate HTRF® Europium cryptate donor / Red acceptor readout. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. • HTRF cAMP HiRange Assay Kit (Cisbio Bioassays, France) • 96-well half area plates (white) (Greiner Bio-One, Germany) • 384-well small volume plates (white and black) (Greiner Bio-One, Germany) HTRF RCK The HTRF RCK is used to confirm the general suitability of a reader for HTRF assays, as well as set up and performance validation. For more information about set-up and compatible HTRF ® readers, please visit our website at: ® pre-mixed antibodies TWO- PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: 25 µL cells . Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Read & analyse. Page 30 4. FOR HTRF CERTIFIED READER For. The EnVision XCite multimode plate reader provides exceptional sensitivity across all detection technologies, while keeping you flexible with monochromator option and full upgradeability. Remove the plate seal and read on an HTRF compatible reader. 5 EXAMPLE OF MAP PLATE . , filters and/or monochromators), a second optical system for the selection of emission light, and a detector, usually one or multiple photomultiplier tubes (PMTs). CLARIOstar is equipped with a specific optical device which enables the measurement of. If not, our scientific team will help you choose the instrument that best suits your needs and budget. 2. It also integrates with the powerful SoftMax Pro Software for. The PHERAstar ® FSX is our most sensitive multi-mode reader for high-throughput screening, combining this sensitivity with fast read times. HTRF Signal + 16 µL 4 µL Standard of pre-mixed IL8 Antibodies or Sample ADD READ ANALYSE Incubate 1 hours* at room temperature *Following incubation, the signal remains stable over a period of 24 hours. Plate 25 μL of cells in. + 4 µL HTRF pre-mixed antibodies. The ratio between control lysate signal / non-specific signal should be greater than 2. Microplate compatibility is a pivotal importance to the optimization and consistent functionality of any plate reader. ONE- PLATE ASSAY PROTOCOL: 5 µL cells + 5 µL compounds (or stimulation buffer) + 5 µL agonist + 5 µL HTRF ® pre-mixed antibodies * Depending on cell lines used, volume of lysis should be optimized. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Plus . pre+ 5 µL HTRFcompounds Activation Buffer. HTRF combines standard FRET technology with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. It features a modular design that allows users to add or change detection cartridges according to their needs. Time-resolved fluorescence was measured on the SpectraMax Multi-Mode Microplate Readers (see Table 2 for instrument settings). FOR HTRF CERTIFIED READERThe assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. FOR HTRF CERTIFIED READER . Plate 25 μL of cells in. Interaction with the industry and research communities is essential for innovating! For more than 30 years, Cisbio has been serving the needs of the clinical and drug discovery communities, delivering a world-class portfolio of assays designed to help find answers faster. + 4 µL HTRF pre-mixed antibodies . For more information. For more information about HTRF. Depending on the cell lines used, it may be necessary to dilute the cell lysate to ensure samples are within the assay linear range. For more information about HTRF. Nephelometers are dedicated instruments that can only detect scattering and are only available as standalone plate readers. Note that any dilution or improper use of d2 and cryptate Tb-detection reagents will impair the assay quality. Certified for use with Alpha, LANCE ® or DELFIA ®. Make sure the setup for Eu3+ Cryptate is used. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. For a list of HTRF-compatible readers and set-up recommendations, please visit . Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Cells in each image are identified by SoftMax Pro Software and cell-by-cellHTRF®-certified, DLReady, and SpectraMax® DuoLuc™-certified. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. For more. For more information about HTRF. + 4 µL HTRF pre-mixed antibodies. + 4 µL HTRF ® pre-mixed antibodies . 10 µL Standard or Sample. The reader adds up to three transfers of assay reagents from a 96- or 384-well source plate to enhance assay design using integrated fluidics. Revvity. Customer Transformation Hub. Make sure the setup for Tb Cryptate is used. LANCE ® Ultra DELFIA ® Certified HTRF Readers and Setups Already more than 50 certified or compatible instruments, and counting! HTRF is compatible with a large. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. 3. The lysis buffer 4X is ready-to-use. Flexibility combined with the best sensitivity of its class, and our newly developed Enhanced Dynamic Range technology, make it the ideal reader for assay development. It can also be further streamlined to TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: Small volume detection plate 96-half well plate. 1, introducing a time delay of approximately 50 to 150 µ seconds between the system excitation and fluorescence measurement allows the signal to be cleared of all non. The optional HTRF-certified filter-based module may be. It accepts all types of microtitration plate. For more information about HTRF. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. According to the manufacturers protocol, this protein assay is linear in the range of 0. HTRF is a versatile TR-FRET technology developed by Cisbio for detecting biomolecular interactions. Lysates are then transferred to the assay plate for the detection of Phospho-VASP (Ser239) by HTRF. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection TWO-PLATE ASSAY PROTOCOL FOR SUSPENSION CELLS: Small volume detection plate 96-half well plate 25 µL cells + 5 µL compounds + 10 µL supplemented lysis buffer (4X)* + 4 µL HTRF® pre-mixed antibodies Read & analyse Incubate 4h at RT Transfer 16 µL supernatant LysisAdd ONE-PLATE ASSAY PROTOCOL: Small volume detection plate 8 µL cells CLARIOstar readers can be equipped with monochromators or filters and only filter version is HTRF certified . HTRF® 96-well low volume plate Ref# 66PL96001 * HTRF® 384-well low volume plate Ref 66PL384025 * Non-binding 96-well black plate HTRF®-Certified Reader **. Choose from over 50 certified or compatible plate readers More than 50 certified or compatible instruments, and counting! Your lab might even already have one. FOR HTRF CERTIFIED READER. MATERIALS PROVIDED: KIT . The EnVision ® multimode plate reader provides exceptional speed, ultra-high throughput, and maximum sensitivity across all detection technologies. (donor) of compounds are measured on an HTRF-compatible reader. The VICTOR ® Nivo ™ system packs all popular detection modes into the industry’s smallest multimode reader. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. Assay setup for a 384-well low-volume plate. NON-SPECIFIC BINDING TOTAL BINDINGCover the plate with a plate sealer. For more information about HTRF. FOR HTRF CERTIFIED READER For more. Plate 25 μL of cells in. Utilizing HTRF (Homogeneous Time-resolved Fluorescence) technology, the assay enables simple and rapid characterization of compound and antibody blockers in a high throughput format. Incubate overnight at RT. Add. Small ONE- PLATE ASSAY PROTOCOL: overnight FOR HTRF CERTIFIED READER . To perform the assay, use white plate only. The kit includes reagents, buffers and a microplate. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. Incubate 2 h at RT. Depending on cell lines used, it can be necessary to dilute the cell lysate to ensure samples are within the assay linear range FOR HTRF CERTIFIED READER. HTRF Signal + 16 µL Standard or Sample 4 µL of pre-mixed TNFα Antibodies ADD READ ANALYSE Incubate 2 hours* at room temperature *Following incubation, the signal remains stable over a period of 24 hours. Plate 50 µL of cells in 96-well tissue-culture treated plate in appropriate growth medium and incubate overnight, at 37°C in CO2 atmosphere. It can also be further streamlined toLysates are then transferred ®to the assay plate for the detection of Total TYK2 by HTRF reagents. Because of its homogeneous and fast nature, the assay is a preferred method to determine the protein concentration of samples. Please note that this kit use a Terbium donor crypate, make sure to set up your reader for Terbium cryptate. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. It features a modular design that allows users to add or change detection cartridges according to their needs. First the plate is coated with a capture antibody. Incubate overnight at RT. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Plate 25 μL of cells in. Conclusion. It continues to deliver optimal results. CLARIOstar is equipped with a specific optical device which enables the measurement of both 620 nm cryptate and 665 nm acceptor emissions. Its. Plate 25 μL of cells in 96 half-well plate in your appropriate medium. HTRF Signal + 2 hours @ RT [Cortisol] ng/ml 10 µL Standard or Sample 5 µL Cortisol acceptor reagent 5 µL Cortisol donor antibody ADD READ & ANALYSESafire 2™ readers must be appropriately be configured for HTRF readout by setting up the measurement conditions in the “multilabeling” function of Magellan software. The assay can be run under a two-plate assay protocol, where cells are plated, stimulated and lysed in the same culture plate. Choose from over 50 certified or compatible plate readers More than 50 certified or compatible instruments, and counting! Your lab might even already have one. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. They can be used in a no-transfer protocol with suspension cells or with a transfer step for adherent cells. The SpectraMax ® iD5 Multi-Mode Microplate Reader is HTRF-certified, with an enhanced TRF detection module and filters optimized for HTRF detection. For more information about HTRF ®• HTRF®-Certified Reader. use of a wide variety of plates or sample matrix formats. The plate was covered and incubated for one hour at room temperature. FOR HTRF CERTIFIED READER. FOR HTRF CERTIFIED READER. 1. But sometimes these parameters are not sufficient enough. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. • HTRF®-Certified Reader. Remove the plate seal and read on an HTRF compatible reader. HTRF is a proprietary time-resolved fluoresce nce technology that overcomes To prevent evaporation during assay incubation steps, we recommend the use of TopSeal-A PLUS, a clear adhesive seal for microplate. The controls are equivalent to the upper and lower limit of. For more information about HTRF ® compatible readers and for set-up recommendations, please. Specialized filters allow for IR fluorescence. Incubate overnight at RT. This protocol gives the cells viability and confluence to be monitored. The PHERAstar FSX is the best microplate reader for all HTRF assays: Sensitive, fast and reliable measurements with best Z´ and DeltaF% HTRF-certified with white and black. Partnerships for delivering turn-key solutions. FOR HTRF CERTIFIED READER For more. No washing steps are required. The SpectraMax i3x and iD5 readers can be equipped with an HTRF-certified detection cartridge or an HTRF-certified detection system, respectively. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for multiuser labs. FOR HTRF CERTIFIED READER For. Microplate reader assays requiring infrared (IR) or red-shifted assays can be read with great sensitivity using the iD5 reader’s filter system. Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized. Figure 3. + 4 µL HTRF ® pre-mixed antibo dies 2. 96-well culture plateONE-PLATE ASSAY PROTOCOL: Small volume detection plate 8 µL cells + 4 µL compounds + 4 µL supplemented ® lysis buffer (4X) + 4 µL HTRF pre-mixed antibodies Read & analyse Incubate overnight at RT Add Lysis Add * Depending on cell lines used, volume of lysis should be optimized.